Fish

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    Protocols in Current Issue
    Inter-species Transplantation of Blastocysts between Medaka and Zebrafish
    Authors:  Jana F. Fuhrmann, Jasmin Onistschenko and Lázaro Centanin, date: 09/20/2021, view: 1259, Q&A: 0
    [Abstract]

    Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where it is typically used for intra-species blastula transplantations – i.e., labeled blastocysts

    ...
    Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
    Authors:  Yu Murakami, Satoshi Ansai, Akari Yonemura and Masato Kinoshita, date: 12/20/2017, view: 6061, Q&A: 0
    [Abstract] This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.



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