Cell Biology


    Protocols in Current Issue
    Preparation of Outer Membrane Vesicles from Myxococcus xanthus
    Authors:  James E. Berleman, Marcin Zemla, Jonathan P Remis and Manfred Auer, date: 01/20/2016, view: 10032, Q&A: 0
    [Abstract] Outer membrane vesicles (OMVs) represent a unique sub-cellular compartment of bacteria that may act as a scaffold for various extracellular activities, including intercellular signaling. Myxococcus xanthus (M. xanthus) is a predatory bacterium that engages in cell-cell behaviors such as fruiting body formation and contact ...
    Separation of Intracellular Vesicles for Immunoassays
    Authors:  Toshihiko Kobayashi, Tsubasa Tanaka and Noriko Toyama-Sorimachi, date: 08/20/2015, view: 10587, Q&A: 0
    [Abstract] The endosome/lysosome systems play important roles in immune cell functions as signaling platforms. Immune cells utilize these endosome/lysosome for signal transduction or intercellular communication to elicit the proper immune responses, regulating the localization or the association of the signaling complexes. Here we introduce the procedures to ...
    Vesicle Isolation from Bacillus subtilis Biofilm
    Authors:  Lisa Brown, Anne Kessler and Arturo Casadevall, date: 03/05/2015, view: 11629, Q&A: 2
    [Abstract] Bacterial biofilms are associated clinically with many bacterial infections including those caused by bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. In recent years, extracellular vesicles produced by bacteria have been isolated from biofilm communities. Vesicles have been described in depth and can ...
    Preparation of Outer Membrane Vesicle from Escherichia coli
    [Abstract] Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. Previous biochemical and proteomic studies have revealed that the Gram-negative bacteria-derived OMVs are composed of various components like outer membrane proteins, ...

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