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One-step Affinity Purification of the Chloroplast ClpP Complex from the Green Alga
Chlamydomonas reinhardtii
Using the Strep-tagII Epitope Tag
BD
Benoit Derrien*
OV
Olivier Vallon
0
Q&A
9243 Views
Jan 5, 2013
This protocol describes the affinity purification of the chloroplast ClpP complex from
Chlamydomonas reinhardtii
. To this purpose, we have created a
Chlamydomonas reinhardtii
strain in which the chloroplast encoded ClpP1 subunit of the ClpP complex is tagged at its C-terminal end by a strep-tagII peptide.
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