Stem Cell


    Protocols in Current Issue
    Isolation and Culture of Human Adipose-derived Stem Cells from Subcutaneous and Visceral White Adipose Tissue Compartments
    [Abstract] Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human ...
    Isolation and Primary Culture of Various Cell Types from Whole Human Endometrial Biopsies
    Authors:  Flavio Santos Vasconcelos Barros, Jan Joris Brosens and Paul John Brighton, date: 11/20/2016, view: 16875, Q&A: 1
    [Abstract] The isolation and primary culture of cells from human endometrial biopsies provides valuable experimental material for reproductive and gynaecological research. Whole endometrial biopsies are collected from consenting women and digested with collagenase and DNase I to dissociate cells from the extracellular matrix. Cell populations are then ...
    PBMC-MSC Co-cultures for Induction of Treg Generation
    Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 13397, Q&A: 1
    [Abstract] To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were co-cultured with allogeneic MSC or CM for one week followed by one week of culture in the absence of MSC.
    Monocyte-MSC Co-cultures
    Authors:  Sara M. Melief, C. L. M. Schrama and Helene Roelofs, date: 01/20/2015, view: 10575, Q&A: 0
    [Abstract] To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage colony-stimulating factor (M-CSF). Monocytes were isolated from peripheral blood mononuclear cell (PBMC) ...
    Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors
    Authors:  Takafumi Yokota and Yusuke Satoh, date: 02/20/2014, view: 9039, Q&A: 0
    [Abstract] This protocol is useful to determine the frequencies of lymphohematopoietic progenitors in tested samples. To effectively support the growth and differentiation of primitive lymphohematopoietic progenitors, complex signals from stromal cells are important. Several stromal cell lines are known to support both lymphoid and myeloid cells ...
    Isolation of Multipotent Stromal Cells from Mouse Bone Marrow
    Authors:  Aurélie Jeanne Tormo, Moutih Rafei and Jean-François Gauchat, date: 02/20/2014, view: 8715, Q&A: 0
    [Abstract] Generating mouse multipotent stromal cells (MSC) from bone-marrow cells is usefull for a wide range of applications. Effectively, these MSC can differentiate into adipocytes, osteocytes [See “Binding to Secreted Bone Matrix in vitro” (Tormo et al., 2014)] or ...
    Binding to Secreted Bone Matrix in vitro
    Authors:  Aurélie Jeanne Tormo, Christian Beauséjour and Jean-François Gauchat, date: 02/20/2014, view: 8459, Q&A: 0
    [Abstract] This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits ...

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