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Pea Aphid Rearing, Bacterial Infection and Hemocyte Phagocytosis Assay

Authors: Li Ma, Lu Liu and Zhiqiang Lu, date: 12/20/2020, view: 1703, Q&A: 0

[Abstract]

Insects rely on the simple but effective innate immune system to combat infection. Cellular and humoral responses are interconnected and synergistic in insects’ innate immune system. Phagocytosis is one major cellular response. It is difficult to collect clean hemolymph from the small insect like pea aphid. Here, we provide a practicable

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Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

Authors: Yanrong You, Chunpeng An and Chuanyou Li, date: 03/05/2020, view: 3141, Q&A: 0

[Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...

Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves

Authors: Rim Hamza, José P. Beltrán and Luis A. Cañas, date: 09/05/2018, view: 5681, Q&A: 0

[Abstract] Enzymes play a key role in insect-plant relationships. For a better understanding of these interactions, we analyzed Tuta absoluta digestive enzymes. Here, we describe a detailed protocol for the detection of trypsin and papain-like enzymes in Tuta absoluta larvae by enzyme histochemistry. This assay uses frozen and unfixed ...

An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues

Authors: Rupesh Kariyat, Jesus Chavana and Jasleen Kaur, date: 08/20/2018, view: 4629, Q&A: 0

[Abstract] Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...

A Reliable Assay to Evaluate the Virulence of Aspergillus nidulans Using the Alternative Animal Model Galleria mellonella (Lepidoptera)

Authors: Caroline Mota Fernandes, Fernanda Lopes Fonseca, Gustavo Henrique Goldman, Marcos Dias Pereira and Eleonora Kurtenbach, date: 06/05/2017, view: 9076, Q&A: 0

[Abstract] The greater wax moth Galleria mellonella has emerged as an effective heterologous host to study fungal pathogenesis and the efficacy of promising antifungal drugs (Mylonakis et al., 2005; Li et al., 2013). Here, a methodology describing the Aspergillus nidulans infection in G. mellonella larvae, along ...

Bioassays to Investigate the Effects of Insect Oviposition on a Plant’s Resistance to Herbivores

Authors: Michele Bandoly and Anke Steppuhn, date: 06/05/2016, view: 11376, Q&A: 0

[Abstract] Plants respond to herbivory with diverse defence responses (Schoonhoven et al., 2005). Many herbivorous insects deposit their eggs on their host plants before their larvae start to feed. Thus, plants could use insect eggs as a signal to increase their resistance to herbivores. Here, we report experimental procedures to explore whether and ...

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