Cell Biology


    Protocols in Current Issue
    Isolation of Cytosol, Microsome, Free Polysomes (FPs) and Membrane-bound Polysomes (MBPs) from Arabidopsis Seedlings
    Authors:  Yonghui Zhao and Shengben Li, date: 08/05/2017, view: 9918, Q&A: 0
    [Abstract] The plant endomembrane system plays vital roles for synthesis, modification and secretion of proteins and lipids. From the classic view, only mRNAs encoding secreted proteins could be targeted to the endoplasmic reticulum (ER) for translation via a co-translational translocation manner, however, recently this model has been challenged by ...
    ER Microsome Preparation and Subsequent IAA Quantification in Maize Coleoptile and Primary Root Tissue
    Author:  Verena Kriechbaumer, date: 05/05/2016, view: 9718, Q&A: 0
    [Abstract] Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the ...
    Microsome Isolation from Tissue
    Authors:  Maria Bodero and Jose Francisco Abisambra, date: 02/05/2014, view: 11217, Q&A: 0
    [Abstract] This protocol details the extraction of microsomes from frozen tissue in order to further examine the protein-protein interactions occurring within the endoplasmic reticulum. This protocol was adapted from Abisambra et al. (2013) with modifications made in order to optimize for subsequent use.
    Stem Microsome Preparation and Mannan Synthase Activity Assay
    Author:  Yan Wang, date: 05/20/2013, view: 7940, Q&A: 0
    [Abstract] Mannans are hemicellulosic polysaccharides and are present in cell walls of all land plants. Mannan polysaccharides are synthesized by two enzymes, mannan synthase (ManS) for backbone (mannan or glucomannan) synthesis and galactomannan galactosyl transferase for side-chain (galactosyl) addition. Here, a method for ManS activity assay using ...

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