Bio-protocol - Improve Research Reproducibility

A Method to Induce Brown/Beige Adipocyte Differentiation from Murine Preadipocytes

Authors: Andréa Livia Rocha, Beatriz Alves Guerra, Jeremie Boucher and Marcelo A. Mori, date: 12/20/2021, view: 1261, Q&A: 0

[Abstract]

Adipocytes exhibit different morphological and functional characteristics, depending on their anatomical location, developmental origin, and stimulus. While white adipocytes tend to accumulate energy as triglycerides, brown and beige adipocytes tend to direct carbon sources to fuel thermogenesis. White and beige adipocytes originate from common

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Treadmill Running of Mouse as a Model for Studying Influence of Maternal Exercise on Offspring

Authors: Song Ah Chae, Jun Seok Son, Mei-Jun Zhu, Jeanene M. de Avila and Min Du, date: 12/05/2020, view: 2275, Q&A: 0

[Abstract]

Epidemiological studies robustly show the beneficial effects of maternal exercise in reducing maternal birth complications and improving neonatal outcomes, though underlying mechanisms remain poorly understood. To facilitate mechanistic exploration, a protocol for maternal exercise of mice is established, with the regimen following the exercise

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Mouse Adipose Tissue Protein Extraction

Authors: Yu A. An and Philipp E. Scherer, date: 06/05/2020, view: 7670, Q&A: 0

[Abstract] As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins from AT is challenging due to abundant fat content of intracellular lipid droplets. Several commercial kits for extraction of AT proteins are ...

Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation

Authors: Gileade P. Freitas, Alann T. P. Souza, Helena B. Lopes, Rayana L. B. Trevisan, Fabiola S. Oliveira, Roger R. Fernandes, Fernanda U. Ferreira, Felipe A. Ros, Marcio M. Beloti and Adalberto L. Rosa, date: 02/20/2020, view: 4892, Q&A: 1

[Abstract] Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and ...

Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells

Authors: Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen, date: 07/20/2019, view: 4574, Q&A: 0

[Abstract] Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...

Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum

Authors: Qian Tang, Yu-Wen Su and Cory J. Xian, date: 06/20/2019, view: 6751, Q&A: 0

[Abstract] It has been well-established that malondialdehyde (MDA), which is generated during the process of lipid peroxidation, is a commonly known biomarker for oxidative stress. Therefore, the serum levels of MDA are detected by using the lipid peroxidation assay with commercially available kit to determine the induction of oxidative stress in rat models.

Insulin Tolerance Test under Anaesthesia to Measure Tissue-specific Insulin-stimulated Glucose Disposal

Authors: Daniel J. Fazakerley, Andreas M. Fritzen, Marin E. Nelson, Ida H. Thorius, Kristen C. Cooke, Sean J. Humphrey, Gregory J. cooney and David E. James, date: 01/20/2019, view: 5729, Q&A: 0

[Abstract] Insulin resistance is a pathophysiological state defined by impaired responses to insulin and is a risk factor for several metabolic diseases, most notably type 2 diabetes. Insulin resistance occurs in insulin target tissues including liver, adipose and skeletal muscle. Methods such as insulin tolerance tests and hyperinsulinaemic-euglycaemic ...

Isolation and Analysis of Stromal Vascular Cells from Visceral Adipose Tissue

Authors: Jessica Vu and Wei Ying, date: 08/20/2017, view: 11428, Q&A: 0

[Abstract] The obesity epidemic is the underlying driver of the type 2 diabetes mellitus epidemic. A remarkable accumulation of various pro-inflammatory immune cells in adipose tissues is a hallmark of obesity and leads to pathogenesis of tissue inflammation and insulin resistance. Here, we describe a detailed protocol to isolate adipose tissue stromal ...

Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue

Authors: Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky, date: 08/20/2017, view: 10837, Q&A: 1

[Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...

Secretion of Adipsin as an Assay to Measure Flux from the Endoplasmic Reticulum (ER)

Authors: Alexandria Brumfield, Natasha Chaudhary and Timothy E. McGraw, date: 04/05/2017, view: 6493, Q&A: 0

[Abstract] In this protocol we describe a quantitative biochemical assay to assess the efficiency of endoplasmic reticulum (ER) to Golgi protein transport in adipocytes (Bruno et al., 2016). The assay takes advantage of the fact that adipocytes secrete various bioactive proteins, known as adipokines. As a measure of ER to Golgi flux we determine the ...

Mammalia

Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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