Cell Biology

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    Protocols in Current Issue
    TRACES: a Freely Accessible, Semi-automated Pipeline for Detection, Tracking, and Quantification of Fluorescently Labeled Cellular Structures
    Authors:  Xueer Jiang, Linhao Jiang and Li-En Jao, date: 08/05/2022, view: 357, Q&A: 0
    [Abstract]

    Subcellular structures exhibit diverse behaviors in different cellular processes, including changes in morphology, abundance, and relative spatial distribution. Faithfully tracking and quantifying these changes are essential to understand their functions. However, most freely accessible methods lack integrated features for tracking multiple

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    Single Molecule Tracking Nanoscopy Extended to Two Colors with MTT2col for the Analysis of Cell-Cell Interactions in Leukemia
    Authors:  Loriane Maillot, Magali Irla and Arnauld Sergé, date: 04/20/2022, view: 1032, Q&A: 0
    [Abstract]

    Single molecule tracking (SMT) is a powerful technique to study molecular dynamics, and is particularly adapted to monitor the motion and interactions of cell membrane components. Assessing interactions among two molecular populations is classically performed by several approaches, including dual-color videomicroscopy, which allows monitoring of

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    Laser Microirradiation and Real-time Recruitment Assays Using an Engineered Biosensor
    Authors:  Carolina dos Santos Passos, Robert E. Cohen and Tingting Yao, date: 03/05/2022, view: 1918, Q&A: 0
    [Abstract]

    Double-strand breaks (DSBs) are lesions in DNA that, if not properly repaired, can cause genomic instability, oncogenesis, and cell death. Multiple chromatin posttranslational modifications (PTMs) play a role in the DNA damage response to DSBs. Among these, RNF168-mediated ubiquitination of lysines 13 or 15 at the N-terminal tail of histone H2A

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    Live-cell Imaging and Analysis of Germline Stem Cell Mitosis in Caenorhabditis elegans
    Authors:  Réda M. Zellag, Yifan Zhao and Abigail R. Gerhold, date: 01/05/2022, view: 1684, Q&A: 0
    [Abstract]

    Model organisms offer the opportunity to decipher the dynamic and complex behavior of stem cells in their native environment; however, imaging stem cells in situ remains technically challenging. C. elegans germline stem cells (GSCs) are distinctly accessible for in situ live imaging but relatively few studies have taken advantage of this

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    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy
    Authors:  Rosa L. López-Marqués and Thomas G. Pomorski, date: 11/20/2021, view: 2067, Q&A: 0
    [Abstract]

    Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable,

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    Live Imaging of Apoptotic Extrusion and Quantification of Apical Extrusion in Epithelial Cells
    Authors:  Kinga Duszyc, Ivar Noordstra, Alpha S. Yap and Guillermo A. Gomez, date: 11/20/2021, view: 1958, Q&A: 0
    [Abstract]

    Apoptotic cell death eliminates unhealthy cells and maintains homeostatic cell numbers within tissues. Epithelia, which serve as fundamental tissue barriers for the body, depend on a physical expulsion of dying cells (apoptotic cell extrusion) to remain sealed and intact. Apoptotic cell extrusion has been widely studied over recent years, with

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    Gamete Fusion Assay in Mice
    Author:  Naokazu Inoue, date: 11/20/2021, view: 1466, Q&A: 0
    [Abstract]

    Gamete fusion, which is the final event of fertilization, is a crucial physiological event in the creation of a new fetus. In mammals, sperm IZUMO1 and oocyte IZUMO1R (JUNO) recognition play a role in triggering this process. Gamete fusion occurs through a complex but steady and unfailing intermolecular reaction because fertilization must ensure

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    Simultaneous Monitoring Cytoplasmic Calcium Ion and Cell Surface Phosphatidylserine in the Necrotic Touch Neurons of Caenorhabditis elegans
    Authors:  Yoshitaka Furuta and Zheng Zhou, date: 10/20/2021, view: 1097, Q&A: 0
    [Abstract]

    Calcium ions trigger many cellular events, including the release of neurotransmitters at the synaptic terminal and excitotoxic cell death. Recently, we have discovered that a transient increase in the level of cytoplasmic Ca2+ triggers the exposure of phosphatidylserine (PS) on the surfaces of necrotic cells in the nematode Caenorhabditis elegans.

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    Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators
    Authors:  Amit Jairaman and Michael D. Cahalan, date: 10/05/2021, view: 1798, Q&A: 0
    [Abstract]

    Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold standard for visualization and quantification of intracellular Ca2+ signals; ratiometric imaging can be

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    In vivo Optical Access to Olfactory Sensory Neurons in the Mouse Olfactory Epithelium
    Authors:  Shigenori Inagaki, Ryo Iwata and Takeshi Imai, date: 06/20/2021, view: 2336, Q&A: 0
    [Abstract]

    In neuroscience, it is fundamental to understand how sensory stimuli are translated into neural activity at the entry point of sensory systems. In the olfactory system, odorants inhaled into the nasal cavity are detected by ~1,000 types of odorant receptors (ORs) that are expressed by olfactory sensory neurons (OSNs). Since each OSN expresses only

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