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Construction of Glycine Oxidase Mutant Libraries by Random Mutagenesis, Site Directed Mutagenesis and DNA Shuffling
Author:
Tao Zhan
,
date:
10/05/2014,
view:
9418,
Q&A:
0
[Abstract] Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the C-N bond in glyphosate (Pedotti
et al.
, 2009). ...
Cytotoxicity Assay for Detection of Cereulide Produced by Emetic
Bacillus cereus
Authors:
Elrike Frenzel
and
Monika Ehling-Schulz
,
date:
01/20/2013,
view:
11692,
Q&A:
0
[Abstract] The emetic subgroup of
Bacillus cereus
strains produces cereulide, a dodecadepsipeptide (1.2 kDa), which is the causative agent of food poisonings. Cereulide is synthesized by a nonribosomal peptide synthetase (NRPS), the Ces-NRPS (Ehling-Schulz
et al.
, 2006). Cereulide is a cyclic and lipophilic potassium ionophor structurally ...
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