Protocols in Current Issue
    Construction of Glycine Oxidase Mutant Libraries by Random Mutagenesis, Site Directed Mutagenesis and DNA Shuffling
    Author:  Tao Zhan, date: 10/05/2014, view: 9418, Q&A: 0
    [Abstract] Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the C-N bond in glyphosate (Pedotti et al., 2009). ...
    Cytotoxicity Assay for Detection of Cereulide Produced by Emetic Bacillus cereus
    Authors:  Elrike Frenzel and Monika Ehling-Schulz, date: 01/20/2013, view: 11692, Q&A: 0
    [Abstract] The emetic subgroup of Bacillus cereus strains produces cereulide, a dodecadepsipeptide (1.2 kDa), which is the causative agent of food poisonings. Cereulide is synthesized by a nonribosomal peptide synthetase (NRPS), the Ces-NRPS (Ehling-Schulz et al., 2006). Cereulide is a cyclic and lipophilic potassium ionophor structurally ...

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