Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
Author: Zhongyi Li,
date: 01/20/2016,
view: 9585,
Q&A: 0
[Abstract] Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at ...
In situ Digestion of Wheat Cell-wall Polysaccharides
Authors: Dušan Veličković and
Hélène Rogniaux,
date: 12/05/2014,
view: 10426,
Q&A: 2
[Abstract] Cell walls of the wheat endosperm are mostly composed of arabinoxylans (AX) and mixed (1→3), (1→4)-β-glucans (BG) (Saulnier et al., 2012). Here, we present an optimized protocol to degrade enzymatically these cell-wall polysaccharides into oligosaccharides, directly from wheat grain cross sections. The main difficulty is to provide a ...