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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
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Isolation of Tumor Cells Based on Their Distance from Blood Vessels
Authors:
Saran Kumar
,
Husni Sharife
,
Tirzah Kreisel
,
Libat Bar-Lev
,
Myriam Grunewald
and
Eli Keshet
,
date:
05/20/2020,
view:
3529,
Q&A:
0
[Abstract] Differential exposure of tumor cells to microenvironmental cues greatly impacts cell phenotypes, raising a need for position based sorting of tumor cells amenable to multiple OMICs and functional analyses. One such key determinant of tumor heterogeneity in solid tumors is its vasculature. Proximity to blood vessels (BVs) profoundly affects tumor ...
A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
Authors:
Chloe-Anne Martinez
,
Peter A. Cistulli
and
Kristina M. Cook
,
date:
09/20/2019,
view:
4726,
Q&A:
0
[Abstract] Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and behavior and pericellular oxygen levels should be controlled when establishing
in vitro
models. ...
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