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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
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Conjugation Protocol Optimised for
Roseburia inulinivorans
and
Eubacterium rectale
Authors:
Paul O. Sheridan
,
Jennifer C. Martin
and
Karen P. Scott
,
date:
04/05/2020,
view:
3177,
Q&A:
0
[Abstract]
Roseburia
and
Eubacterium
species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
Delivering "Chromatic Bacteria" Fluorescent Protein Tags to Proteobacteria Using Conjugation
Authors:
Rudolf O Schlechter
and
Mitja NP Remus-Emsermann
,
date:
04/05/2019,
view:
5783,
Q&A:
0
[Abstract] Recently, we published a large and versatile set of plasmids, the chromatic bacteria toolbox, to deliver eight different fluorescent protein genes and four combinations of antibiotic resistance genes to Gram-negative bacteria. Fluorescent tags are important tools for single-cell microbiology, synthetic community studies, biofilm, and host-microbe ...
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