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An Assay to Determine NAD(P)H: Quinone Oxidoreductase Activity in Cell Extracts from Candida glabrata

Authors: Anamika Battu, Rajaram Purushotham and Rupinder Kaur, date: 11/05/2021, view: 1012, Q&A: 0

[Abstract]

Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans. These proteins possess NAD(P)H:quinone oxidoreductase activity and convert quinones to hydroquinones through two-electron reduction, using NAD(P)H and quinone as electron donor

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Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata

Authors: Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 9317, Q&A: 0

[Abstract] Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was ...

Genetic Transformation of Candida glabrata by Heat Shock

Authors: Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 8028, Q&A: 0

[Abstract] Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogen ...

Genetic Transformation of Candida glabrata by Electroporation

Authors: Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 7276, Q&A: 0

[Abstract] Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata ...

Catalase Activity Assay in Candida glabrata

Authors: Emmanuel Orta-Zavalza, Marcela Briones-Martin-del-Campo, Irene Castano and Alejandro De Las Penas, date: 03/20/2014, view: 17573, Q&A: 1

[Abstract] Commensal and pathogenic fungi are exposed to hydrogen peroxide (H₂O₂) produced by macrophages of the host. Pathogenic fungi counteract the harmful effects of H₂O₂ with the enzyme catalase (EC 1.11.1.6), which decomposes two molecules of H₂O₂ to two molecules of H₂O and O ...

Minimum Inhibitory Concentration (MIC) Assay for Antifungal Drugs

Authors: Jinglin L. Xie, Sheena D. Singh-Babak and Leah E. Cowen, date: 10/20/2012, view: 32165, Q&A: 0

[Abstract] The Minimum Inhibitory Concentration (MIC) Assay is widely used to measure the susceptibility of yeasts to antifungal agents. In serial two-fold dilutions, the lowest concentration of antifungal drug that is sufficient to inhibit fungal growth is the MIC. Typically, 50% inhibitory (MIC₅₀) or 80% inhibitory (MIC₈₀) values are ...

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