Microbiology

Categories

    Protocols in Current Issue
    A Rapid Induction Overexpression System for the Fission Yeast Schizosaccharomyces pombe
    Author:  Angad Garg, date: 10/20/2021, view: 774, Q&A: 0
    [Abstract]

    The fission yeast Schizosaccharomyces pombe is an excellent genetically tractable model organism used in the study of conserved eukaryotic cellular biology. One genetic tool in the assessment of gene function is the in vivo overexpression of proteins. Existing overexpression tools have limitations of induction kinetics, dynamic range, and/or

    ...
    Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale
    Authors:  Paul O. Sheridan, Jennifer C. Martin and Karen P. Scott, date: 04/05/2020, view: 3190, Q&A: 0
    [Abstract] Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
    Evaluation of Plasmid Stability by Negative Selection in Gram-negative Bacteria
    Authors:  Damián Lobato Márquez and Laura Molina García, date: 05/05/2017, view: 10692, Q&A: 0
    [Abstract] Plasmid stability can be measured using antibiotic-resistance plasmid derivatives by positive selection. However, highly stable plasmids are below the sensitivity range of these assays. To solve this problem we describe a novel, highly sensitive method to measure plasmid stability based on the selection of plasmid-free cells following elimination ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.