Neuroscience

Categories

    Protocols in Current Issue
    Vestibular Organ Dissection and Whole-Mount Immunolabeling in Mouse
    Authors:  Timothy S. Balmer and Laurence O. Trussell, date: 05/20/2022, view: 414, Q&A: 0
    [Abstract]

    The vestibular sensory apparatus contained in the inner ear is a marvelous evolutionary adaptation for sensing movement in 3 dimensions and is essential for an animal’s sense of orientation in space, head movement, and balance. Damage to these systems through injury or disease can lead to vertigo, Meniere’s disease, and other disorders

    ...
    Multiplexing Thermotaxis Behavior Measurement in Caenorhabditis elegans
    Authors:  Stephan Raiders, Mason Klein and Aakanksha Singhvi, date: 04/05/2022, view: 853, Q&A: 0
    [Abstract]

    Thermotaxis behaviors in C. elegans exhibit experience-dependent plasticity of thermal preference memory. This behavior can be assayed either at population level, on linear temperature gradients, or at the individual animal level, by radial isothermal or microfluidic tracking of orientation. These behaviors are low-throughput as well as

    ...
    Operant Self-medication for Assessment of Spontaneous Pain Relief and Drug Abuse Liability in Mouse Models of Chronic Pain
    [Abstract]

    The search for safe and efficient chronic pain treatments is dampened by the lack of reliable models that faithfully reproduce current pharmacological treatments for chronic spontaneous pain in humans. Preclinical models often assess the antinociceptive efficacy of non-contingent pharmacological treatments evaluated in the short-term. Here, we

    ...
    Patch-clamp Recordings and Single Fiber Labeling from Spiral Ganglion Somata in Acutely Prepared Semi-intact Cochleae from Neonatal Rats
    Authors:  Alexander L. Markowitz, Megana R. Iyer and Radha Kalluri, date: 01/05/2022, view: 1501, Q&A: 0
    [Abstract]

    Spiral ganglion neurons (SGN) are the primary neuronal pathway for transmitting sensory information from the inner ear to the brainstem. Recent studies have revealed significant biophysical and molecular diversity indicating that auditory neurons are comprised of sub-groups whose intrinsic properties contribute to their diverse functions. Previous

    ...
    Assessment of Corticospinal Excitability in Awake Rodents Using EMG-Controlled Intracortical Stimulation
    Authors:  Windsor K. C. Ting, Derek Burns, Maxime Huot-Lavoie and Christian Ethier, date: 12/20/2021, view: 702, Q&A: 0
    [Abstract]

    Assessment of corticospinal excitability (CSE) is an essential component of experiments designed to induce or study neuronal plasticity in the motor system. Common examples are paired associative stimulation (PAS), theta-burst stimulation (TBS), intensive motor training, or any methods aimed at potentiating the corticomotor system in the hope of

    ...
    Visual-stimuli Four-arm Maze test to Assess Cognition and Vision in Mice
    [Abstract]

    Visual impairments, notably loss of contrast sensitivity and color vision, were documented in Alzheimer’s disease (AD) patients yet are critically understudied. This protocol describes a novel visual-stimuli four-arm maze (ViS4M; also called visual x-maze), which is a versatile x-shaped maze equipped with spectrum- and intensity-controlled

    ...
    Whole-mount Staining of Mouse Diaphragm Neuromuscular Junctions
    Authors:  Rula Sha, Zhenzhen Wang, Xue You, Yuguo Liu, Zhiqin Xie and Ying Feng, date: 11/05/2021, view: 1240, Q&A: 0
    [Abstract]

    The neuromuscular junction (NMJ) is a specialized synapse that connects the terminal end of a motor neuron and a skeletal muscle fiber. Defects in NMJ cause abnormalities of neuromuscular transmission, leading to NMJ disorders. The mammalian diaphragm muscle is essential for respiration and has been widely used to study NMJ formation. Here, we

    ...
    Isolation, Culture, and Identification of Primary Müller Cells from Human Retina
    Authors:  Yingying Chen, Ting Zhang, Shaoxue Zeng, Michelle Yam, Mark C. Gillies and Ling Zhu, date: 10/05/2021, view: 1408, Q&A: 0
    [Abstract]

    Müller cells, the major glial cells of the retina, play vital roles in maintaining redox homeostasis and retinal metabolism. An immortalized human Müller cell line (MIO-M1) is widely used as an in vitro model to study Müller cells’ function, but they may not be exactly the same as primarily cultured human Müller cells. The use of human

    ...
    An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice
    Authors:  David A. Valientes, Anthony M. Raus and Autumn S. lvy, date: 07/05/2021, view: 1931, Q&A: 0
    [Abstract]

    Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived neurotrophic factor (BDNF) in neural plasticity and cognition, have been made using rodents housed with

    ...
    Evaluating Baseline and Sensitised Heat Nociception in Adult Drosophila
    Authors:  Josephine N. Massingham, Olga Baron and G. Gregory Neely, date: 07/05/2021, view: 1502, Q&A: 0
    [Abstract]

    Chronic pain is a complex disease that affects a large proportion of the population. With little to no effective treatments currently available for patients, this malady presents a large burden to society. Drosophila melanogaster has been previously used to describe conserved molecular components of nociception in larvae and adults. However, adult

    ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.