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Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
Authors:
Yusuke Tomina
and
Daniel A. Wagenaar
,
date:
03/05/2018,
view:
6225,
Q&A:
0
[Abstract] In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging. The former entails the removal of the sheath that covers neurons in the ...
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