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Selection of Genetically Modified Bacteriophages Using the CRISPR-Cas System
Authors:
Miriam Manor
and
Udi Qimron
,
date:
08/05/2017,
view:
9555,
Q&A:
0
[Abstract] We present a CRISPR-Cas based technique for deleting genes from the T7 bacteriophage genome. A DNA fragment encoding homologous arms to the target gene to be deleted is first cloned into a plasmid. The T7 phage is then propagated in
Escherichia coli
harboring this plasmid. During this propagation, some phage genomes undergo homologous ...
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