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Serial Cryomicrotomy of
Saccharomyces cerevisiae
for Serial Electron Cryotomography
Authors:
Cai Tong Ng
,
Mark S. Ladinsky
and
Lu Gan
,
date:
11/20/2020,
view:
2450,
Q&A:
0
[Abstract] Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules
in situ
. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be thin. For frozen-hydrated cells, such samples can be produced either by cryomicrotomy or ...
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