Microbiology

Categories

    Protocols in Current Issue
    Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria
    Authors:  Soumila Mondal and Shailendra P. Singh, date: 05/20/2022, view: 39, Q&A: 0
    [Abstract]

    Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the fitness, survival, and growth of cyanobacteria in the laboratory as well as in large scale cultivation

    ...
    Analysis of Heterocyst and Akinete Specific Glycolipids in Cyanobacteria Using Thin-layer Chromatography
    Authors:  Ritu Garg, Rebeca Perez and Iris Maldener, date: 03/20/2022, view: 525, Q&A: 0
    [Abstract]

    Several filamentous cyanobacteria like Nostoc differentiate specialized cells in response to changes in environmental factors, such as low light or nutrient starvation. These specialized cells are termed heterocysts and akinetes. Under conditions of nitrogen limitation, nitrogen-fixing heterocysts form in a semi-regular pattern and provide the

    ...
    High-throughput Growth Measurements of Yeast Exposed to Visible Light
    Authors:  Katarina Logg, Mikael Andersson, Anders Blomberg and Mikael Molin, date: 01/20/2022, view: 872, Q&A: 0
    [Abstract]

    Light is a double-edged sword: it is essential for life on the planet but also causes cellular damage and death. Consequently, organisms have evolved systems not only for harvesting and converting light energy into chemical energy but also for countering its toxic effects. Despite the omnipresence and importance of such light-dependent effects,

    ...
    An Assay to Determine NAD(P)H: Quinone Oxidoreductase Activity in Cell Extracts from Candida glabrata
    Authors:  Anamika Battu, Rajaram Purushotham and Rupinder Kaur, date: 11/05/2021, view: 999, Q&A: 0
    [Abstract]

    Flavodoxin-like proteins (Fld-LPs) are an important constituent of the oxidative stress defense system in several organisms and highly conserved from bacteria to humans. These proteins possess NAD(P)H:quinone oxidoreductase activity and convert quinones to hydroquinones through two-electron reduction, using NAD(P)H and quinone as electron donor

    ...
    Determination of Fungal Tolerance Index to Heavy Metals and Heavy Metal Resistance Tests
    Author:  Rosa Paulina Calvillo-Medina, date: 11/05/2021, view: 933, Q&A: 0
    [Abstract]

    Fungal metallo-tolerance has been described in different species and plays an important role in bioremediation of contaminated environments. Metallo-tolerance is mainly documented by microdilution assays and agar well diffusion methods using equipment that can be expensive. The tolerance index can be calculated to determine the efficiency of a

    ...
    Quantification of RuBisCO Expression and Photosynthetic Oxygen Evolution in Cyanobacteria
    Authors:  Mateusz Kędzior and Betul Kacar, date: 10/20/2021, view: 971, Q&A: 0
    [Abstract]

    Phototrophic microorganisms are frequently engineered to regulate the expression and the activity of targeted enzymes of interest for specific biotechnological and agricultural applications. This protocol describes a method to evaluate the expression of RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) in the model cyanobacterium

    ...
    Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays
    Authors:  Aryashree Arunima and Mrutyunjay Suar, date: 09/20/2021, view: 1018, Q&A: 0
    [Abstract]

    Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation, low micronutrient availability, and toxicity from bile salts, to promote bacterial survival and

    ...
    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    Authors:  Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger, date: 08/20/2021, view: 992, Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

    ...
    Ion Transport Activity Assay for Microbial Rhodopsin Expressed in Escherichia coli Cells
    Authors:  Masae Konno, Keiichi Inoue and Hideki Kandori, date: 08/05/2021, view: 1372, Q&A: 0
    [Abstract]

    Microbial rhodopsins have diverse functions, including roles as light-driven ion pumps, light-gated ion channels, photosensors, and light-regulated enzymes. As the number of rhodopsin-like genes identified has increased in recent years, so has the requirement for rapid identification of their functions. The patch-clamp method is often used to

    ...
    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    Authors:  Amy Bergmann and Zhicheng Dou, date: 06/20/2021, view: 1828, Q&A: 0
    [Abstract]

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

    ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.