Prokaryotes

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    Protocols in Current Issue
    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    Authors:  Julie C. Caruana, Scott N. Dean and Scott A. Walper, date: 09/05/2021, view: 1773, Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

    ...
    Purification of a Protein Exhibiting Isoleucine 2-epimerase Activity from Lactobacillus otakiensis JCM 15040
    Authors:  Yuta Mutaguchi and Toshihisa Ohshima, date: 10/20/2015, view: 6809, Q&A: 0
    [Abstract] Prominent accumulation of D-leucine, D-allo-isoleucine and D-valine was observed in the culture medium of the heterofermentative bacterial species, Lactobacillus otakiensis (L. otakiensis) JCM 15040. The racemase enzyme that resulted in this accumulation, isoleucine 2-epimerase, was purified from the bacterial cells. ...
    Intracellular Glycogen Assays
    Authors:  Yong Jun Goh and Todd R. Klaenhammer, date: 06/05/2014, view: 12032, Q&A: 0
    [Abstract] Glycogen, a soluble multi-branched glucose homopolysaccharide, is composed of chains of α-1,4-linked glucose residues interconnected by α-1,6-linked branches. The classical biosynthetic pathway involves phosphoglucomutase (Pgm), glucose-1-phosphate adenylyltransferase (GlgC or GlgCD), glycogen synthase (GlgA) and branching enzyme (GlgB). ...



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