Cell Biology

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    Protocols in Current Issue
    Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells
    Authors:  Anh H. Le and Laura M. Machesky, date: 04/05/2022, view: 717, Q&A: 0
    [Abstract]

    Macropinocytosis is an evolutionarily conserved process, which is characterized by the formation of membrane ruffles and the uptake of extracellular fluid. We recently demonstrated a role for CYFIP-related Rac1 Interactor (CYRI) proteins in macropinocytosis. High-molecular weight dextran (70kDa or higher) has generally been used as a marker for

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    Rapid Lipid Quantification in Caenorhabditis elegans by Oil Red O and Nile Red Staining
    [Abstract]

    The ability to stain lipid stores in vivo allows for the facile assessment of metabolic status in individuals of a population following genetic and environmental manipulation or pharmacological treatment. In the animal model Caenorhabditis elegans, lipids are stored in and mobilized from intracellular lipid droplets in the intestinal and

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    Optimization of Extracellular Flux Assay to Measure Respiration of Anchorage-independent Tumor Cell Spheroids
    [Abstract] Three-dimensional (3D) cell culture models are widely used in tumor studies to more accurately reflect cell-cell interactions and tumor growth conditions in vivo. 3D anchorage-independent spheroids derived by culturing cells in ultra-low attachment (ULA) conditions is particularly relevant to ovarian cancer, as such cell clusters are ...
    Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein
    Authors:  Waleska Dornas and Detlef Schuppan, date: 12/20/2021, view: 1224, Q&A: 0
    [Abstract]

    Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting

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    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    Authors:  Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden, date: 10/05/2021, view: 2061, Q&A: 0
    [Abstract]

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and targeting metabolic pathways can provide new therapeutic options. Cell metabolism is mostly studied in

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    OrganoPlate Micro-fluidic Microvessel Culture and Analysis
    Authors:  Abidemi Junaid and Thomas Hankemeier, date: 07/05/2021, view: 2502, Q&A: 0
    [Abstract]

    The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

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    Intestinal Co-culture System to Study TGR5 Agonism and Gut Restriction
    Authors:  Snehal N. Chaudhari and A. Sloan Devlin, date: 03/20/2021, view: 3601, Q&A: 1
    [Abstract]

    The activation of the Takeda G-protein receptor 5 (TGR5, also known as the G protein-coupled bile acid receptor 1, GPBAR1) in enteroendocrine L-cells results in secretion of the anti-diabetic hormone Glucagon-Like Peptide 1 (GLP-1) into systemic circulation. Consequently, recent research has focused on identification and development of TGR5

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    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    Authors:  Annika Zink, Pawel Lisowski and Alessandro Prigione, date: 03/05/2021, view: 3971, Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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    Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
    Authors:  Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu, date: 02/20/2021, view: 3111, Q&A: 0
    [Abstract]

    Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

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    Immunoprecipitation of Acetyl-lysine and Western Blotting of Long-chain acyl-CoA Dehydrogenases and Beta-hydroxyacyl-CoA Dehydrogenase in Palmitic Acid Treated Human Renal Tubular Epithelial Cells
    Authors:  Tingting Lv, Suwei Zhu, Yuan Ma, Hong Feng and Qiang Wan, date: 09/20/2020, view: 2986, Q&A: 0
    [Abstract] As one of the main energy metabolism organs, kidney has been proved to have high energy requirements and are more inclined to fatty acid metabolism as the main energy source. Long-chain acyl-CoA dehydrogenases (LCAD) and beta-hydroxyacyl-CoA dehydrogenase (beta-HAD), key enzymes involved in fatty acid oxidation, has been identified as the ...



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