Plant Science

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    Protocols in Current Issue
    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    Authors:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda, date: 06/20/2022, view: 954, Q&A: 0
    [Abstract]

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial in the regulation of both ROS-producing and ROS-consuming systems in plants. NAD content is a

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    A Quick Method to Quantify Iron in Arabidopsis Seedlings
    Authors:  Chandan Kumar Gautam, Huei-Hsuan Tsai and Wolfgang Schmidt, date: 03/05/2022, view: 1294, Q&A: 0
    [Abstract]

    Iron (Fe) is an indispensable micronutrient for plant growth and development. Since both deficiency, as well as a surplus of Fe, can be detrimental to plant health, plants need to constantly tune uptake rates to maintain an optimum level of Fe. Quantification of Fe serves as an important parameter for analyzing the fitness of plants from different

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    Rhizoctonia solani Infection Assay of Young Sugar Beet and Arabidopsis plantlets
    Authors:  Fredrik Dölfors, Louise Holmquist, Georgios Tzelepis and Christina Dixelius, date: 01/20/2022, view: 1443, Q&A: 0
    [Abstract]

    Rhizoctonia solani is a soil-borne fungus, which rarely produces any spores in culture. Hence, all inoculation procedures are based on mycelia, often as a coat on cereal kernels, placed in close vicinity to the plant to be infected. In this protocol, an inoculation method is described where the fungus is first allowed to infest a perlite-maize

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    Proteoliposomes for Studying Lipid-protein Interactions in Membranes in vitro
    Author:  Helmut Kirchhoff, date: 10/20/2021, view: 1030, Q&A: 0
    [Abstract]

    Lipids in biomembranes can control the structure and, therefore, the functionality of membrane-embedded protein complexes. Unraveling how the lipid composition determines the mode of operation of membrane proteins provides mechanistic insights into their functionality. We applied a proteoliposome technique for studying how proteins function in

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    High-throughput Screening for Defense Priming-inducing Compounds in Parsley Cell Cultures
    Authors:  Kathrin Schmitz, Linda Werner and Uwe Conrath, date: 10/20/2021, view: 955, Q&A: 0
    [Abstract]

    Defense priming describes the enhanced potency of cells to activate defense responses. Priming accompanies local and systemic immune responses and can be triggered by microbial infection or upon treatment with certain chemicals. Thus, chemically activating defense priming is promising for biomedicine and agriculture. However, test systems for

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    Dark Respiration Measurement from Arabidopsis Shoots
    Authors:  Jose P. Fonseca, Marcus Griffiths, Larry M. York and Kirankumar S. Mysore, date: 10/05/2021, view: 1826, Q&A: 0
    [Abstract]

    Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and convert glucose molecules from cytoplasmatic glycolysis and oxygen into carbon dioxide and water,

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    Authors:  Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho, date: 08/20/2021, view: 1546, Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    Authors:  Wenjia Yu and Alberto P. Macho, date: 08/05/2021, view: 2013, Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    Authors:  Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang, date: 07/05/2021, view: 2448, Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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    Micrografting in Arabidopsis Using a Silicone Chip
    Authors:  Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi, date: 06/20/2021, view: 3424, Q&A: 1
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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