Protocols in Current Issue
    In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
    [Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
    Root-knot Nematode Penetration and Sclareol Nematicidal Activity Assays
    Authors:  Taketo Fujimoto, Takayuki Mizukubo, Hiroshi Abe and Shigemi Seo, date: 06/20/2016, view: 9107, Q&A: 0
    [Abstract] Plant parasitic nematodes parasitize roots and/or stems of various plants thereby inhibiting absorption of nutrients and moisture. In particular, root-knot nematodes (RKN) are a group of the most devastating pests. Various techniques, such as soil sterilization, cultivation of resistant crops, and chemical application, have been developed to ...
    RNA Isolation From Meloidogyne Spp. Galls
    Authors:  Alejandra García, Marta Barcala, Javier Cabrera, Carmen Fenoll and Carolina Escobar, date: 09/05/2013, view: 8916, Q&A: 0
    [Abstract] We describe an efficient method to obtain a sufficient quantity of RNA from nematode-induced galls with a high quality and integrity, proved to be appropriate for transcriptomic analysis, i.e. real time PCR, microarray hybridization or second generation sequencing. This protocol is efficient for small quantities of galls (organs with high ...

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