After treatment, liver tumors of each group were harvested and weighed. Then, part of liver tumor tissues was fixed with 4% paraformaldehyde (PFA) (Thermo Fisher Scientific, Shanghai, China), dehydrated by gradient ethanol (Sigma, Shanghai, China) and embedded by paraffin. Subsequently, tissues were cut into 5-μm section, dewaxed using xylene (Sigma, Shanghai, China), rehydrated with gradient ethanol (Sigma, Shanghai, China), and stained with hematoxylin and eosin (Nanjing Jiancheng, Nanjing, Jiangsu, China) according to manufacturer’s protocols. Then, the pathological features of liver tumor tissues were analyzed under the light microscope.

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