The hot cold plate was used as a measure of thermal nociceptive responsivity (Woolfe and MacDonald 1944; Bennett and Xie 1988; Le Bars et al. 2001). The apparatus consisted of a circular, temperature-controlled plate, enclosed with a transparent cylinder. At each time, the animal was habituated to the apparatus for 2 consecutive days before being tested the following day. On habituation days, the animal was placed on the room-temperature plate for 2 min, then returned to its’ home-cage. On testing day, the plate was first set to hot (52 °C), the animal was placed inside, and latency to react was recorded. The animal was immediately removed from the plate and returned to its home-cage once it reacted. The animal was then left undisturbed for >1 h, following which the same process was repeated with the plate set to cold (2 °C). Greater latency to react was indicative of increased thermal nociceptive thresholds.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.