The hot cold plate was used as a measure of thermal nociceptive responsivity (Woolfe and MacDonald 1944; Bennett and Xie 1988; Le Bars et al. 2001). The apparatus consisted of a circular, temperature-controlled plate, enclosed with a transparent cylinder. At each time, the animal was habituated to the apparatus for 2 consecutive days before being tested the following day. On habituation days, the animal was placed on the room-temperature plate for 2 min, then returned to its’ home-cage. On testing day, the plate was first set to hot (52 °C), the animal was placed inside, and latency to react was recorded. The animal was immediately removed from the plate and returned to its home-cage once it reacted. The animal was then left undisturbed for >1 h, following which the same process was repeated with the plate set to cold (2 °C). Greater latency to react was indicative of increased thermal nociceptive thresholds.

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