The plantar incision surgery was performed on p45 as described by Brennan et al. (1996). Briefly, animals were anesthetized with 5% isoflurane at 1 L/min of O2. Once induced, animals were transferred to a nose-cone with 2% isoflurane at 1 L/min of O2 for the duration of the procedure. The left hind paw was sterilized with chlorhexidine and alcohol, following which a ~1 cm longitudinal incision was made in the skin from the mid-heel to the first foot pad. A second longitudinal incision was made in the underlying plantaris muscle. The incision was closed with two simple interrupted sutures. A subcutaneous injection of paracetamol (200 mg/kg) was given for postoperative analgesia. Isoflurane was turned off, and animals were allowed to recover in a clean, empty, and half-heated cage. The animal was returned to its’ home-cage once conscious and recovered. For the sham injury, animals were anesthetized with 5% isoflurane at 1 L/min of O2 and then allowed to recover in a clean, empty, half-heated cage, and then returned to their home-cage.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.