On the occasion of obtaining NPS-2, a blood sample (BS-1) was collected from each person enrolled in the study. A second blood sample (BS-2) was subsequently collected two weeks after BS-1.

Serum was prepared from the blood samples for antibody detection. Detection of anti-nucleocapsid (anti-N) and anti-spike (anti-S) IgG antibodies was performed using the commercial assays Elecsys®® Anti-SARS-CoV-2 and Elecsys®® Anti-SARS-CoV-2 S, respectively, in a cobas e 602 module (Roche, Basel, Switzerland).

With regard to anti-N antibodies, values for the ratio S/Co < 1 were considered to be negative and values ≥ 1 were considered to be positive. With regard to anti-S antibodies, values < 0.8 μL−1 were considered to be negative and values ≥ 0.8 μL−1 were considered to be positive.

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