Statistical analyses

SB Scott C. Burgess EJ Erika C. Johnston AW Alex S. J. Wyatt JL James J. Leichter PE Peter J. Edmunds

This protocol is extracted from research article:

Response diversity in corals: hidden differences in bleaching mortality among cryptic Pocillopora species

**
Ecology**,
Jun 7, 2021;
DOI:
10.1002/ecy.3324

Response diversity in corals: hidden differences in bleaching mortality among cryptic Pocillopora species

DOI:
10.1002/ecy.3324

Procedure

The effects of colony size on the probability of bleaching and the probability of whole colony mortality were estimated using binomial generalized linear models. For colonies that were not genetically identified, the effects of colony size on the probability of bleaching and the probability of mortality were compared across sites. For colonies that were genetically identified, sites were pooled for analyses because there were not enough genetically identified samples at each site to directly estimate the interactive effects of site and haplotype identity on the probability of colony mortality (see Appendix S1: Fig. S4 for analyses at Site 2). Each colony was assigned a category of 0 for alive, or 1 for dead. We modelled the probability of bleaching using two approaches. The first approach follows that used in multinomial models. For each bleaching severity category (*i* = 1, 2, and 3), we modelled the probability of bleaching as the log of the odds of bleaching in category *i* vs. not bleaching (*i* = 0) as: $\mathrm{ln}\left[P,\left(\mathrm{bleaching},\phantom{\rule{0.166667em}{0ex}},i\right),/,P,\left(\mathrm{bleacing},\phantom{\rule{0.166667em}{0ex}},0\right)\right]$. In the second approach, we simply distinguished between those corals that did (*i* = 1) or did not (*i* = 0) bleach. Since colony size was only estimated on a subset of colonies for which bleaching and mortality were recorded, the effects of site on bleaching and mortality included a larger sample of colonies than the effects of colony size on bleaching and mortality. The effects of colony size were tested using χ^{2} log‐likelihood ratio tests of models with and without the slope coefficient. Spatial and temporal variation in the relative abundance of haplotypes was assessed using Principal Coordinates Analysis.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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