4.4. Effects of Photosynthetic Electron Transport Inhibitors (Experiment III)

Four photosynthetic inhibitors, diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], atrazine, dibromothymoquinone (DBMIB) and paraquat (N, N′-dimethyl-4,4′-bipyridinium dichloride), were used in Experiment III. Acetone-dissolved diuron, atrazine and DBMIB and water-dissolved paraquat were added to C. marina or C. ovata 5-day cultures in exponential growth stage a final concentration of 0.075, 0.05, 0.05 and 7.5 mg L−1, respectively. A negative control was established by culturing algae with the inhibitors in their original solvents. i.e., acetone for diuron, atrazine for DBMIB and distilled water for paraquat. All cultures were run in triplicate and grown at 24 °C, salinity of 28 and 100 µmol m−2 s−1 light intensity. Hemolytic activity, Fv/Fm, (quantum yield) and rETR were measured after 1 h-incubation; ROS were measured under diuron and DBMIB exposure conditions.

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