4.2. Effects of Light and Iron (Experiment I)

Five different light intensities, 30, 60, 100, 180 and 270 µmol m−2 s1, and three different FeCl3 concentrations: 0, 0.12, and 11.6 µmol L−1, were tested separately for C. marina and C. ovata in Experiment I. Chattonella marina and C. ovata were each incubated in artificial seawater with f/2-Si medium and preconditioned for two generations [106]. All treatments were conducted in triplicate. Samples for hemolytic activity, ROS, cell density and photosynthetic fluorescence parameters were collected every 2 or 3 days.

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