To exploit the photothermal cytotoxicity of BPQDs and BBPQDs, 4T1 cells (1 × 104 cells per well) were seeded into 96-well plates and incubated at 37 °C for 24 h. Different concentrations of BPQDs and BBPQDs were then added into the wells, incubated with cells for 4 h, and then irradiated by the NIR laser (808 nm, 1.0 W/cm2) for 5 min. The cells were then incubated for another 24 h. Cell viability was determined using a MTT assay according to the manufacturer’s protocol.

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