2.7. Ex Vivo Permeation Steady-State Flux across the Membrane (Jss)
This protocol is extracted from research article:
Lyophilized Composite Loaded with Meloxicam-Peppermint oil Nanoemulsion for Periodontal Pain
Polymers (Basel), Jul 14, 2021; DOI: 10.3390/polym13142317

Ex vivo permeability studies for MX for the different MX-PO-SNEDDS formulations were performed on sheep buccal mucosa. The prepared mucosa (2 cm × 2 cm) was mounted on an automatic Franz diffusion cell (MicroettePlus, Hanson Research, Los Angeles, CA, USA) with an area of 1.76 cm2 and receptor cell volume of 7 mL. A known quantity of each formulation (equivalent to 7.5 mg MX) was loaded in the donor part. Phosphate buffered saline, at pH 6.8 and temperature of 32 ± 2 °C under stirring at 400–420 rpm, was employed as the receptor medium. The MX content in the samples withdrawn at predetermined time points was estimated using a high-performance liquid chromatography. The amount of MX permeation per unit area across the sheep buccal mucosa was determined and used to calculate the steady-state flux (Jss).

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