The concentration of sVEGFR1 in colorectal tissue extracts was determined by a human VEGFR1 ELISA kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. The lower detection limit of sVEGFR1 was 0.16 ng/mL. Samples were diluted 1:16.7. The concentration of GrK in colorectal tissue extracts was measured using a human GrK ELISA kit (Aviva Systems Biology) according to the manufacturer’s protocol. The lower detection limit of GrK was 0.03 ng/mL. Samples were diluted 1:4.

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