HUVEC cells were pelleted by centrifugation at 2000 rpm for 5 min before harvesting the conditioned medium. sVEGFR1 levels were measured in a Luminex assay, using the human anti-VEGFR1 antibody (clone 49560, R&D Systems). Measurements were performed using an in-house developed and validated (ISO9001 certified) multiplex immunoassay based on Luminex xMAP technology (Luminex, Austin TX USA) as described (26).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.