The extract of R. adamsii (July sample, 100 mg) was sonically dissolved in 40% methanol (5 mL), centrifuged (6000× g, 20 min), and used for HPLC-PAD separation without SPE pretreatment. The aliquote of R. adamsii extract solution (50 μL) was mixed with 0.5% DPPH radicals solution in methanol (50 μL) and incubated 15 min at 20 °C. The probe without DPPH radicals preincubation was diluted with methanol (1:1) before separation. High-performance liquid chromatography with UV detection coupled with DPPH radicals preincubation performed by microcolumn liquid chromatography MiliChrom A-02 (EcoNova, Novosibirsk, Russia). The apparatus was coupled with UV detector 190-360 EcoNova (EcoNova, Novosibirsk, Russia) and ProntoSIL-120-5-C18 AQ column (50 × 1 mm, 1 μm; Metrohm AG, Herisau, Switzerland) with column temperature of 30 °C. The eluent composition was 0.2 M LiClO4 in 0.01 M HClO4 for eluent A and 0.01 M HClO4 in MeCN for eluent B. The injection volume was 1 μL, and the elution flow was 150 μL/min. The gradient separation was used programmed as 0.0–26.6 min 5–100% B, 26.6–28.6 min 100% B. The chromatograms were recorded at 270 nm. Finally, the chromatograms of untreated and DPPH radicals probes were overlapped and compared. The reduction of chromatographic peak area indicating the radical-scavenging potential of compounds eluted in corresponding peaks.

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