The quantitative analysis of compounds found in R. adamsii was done using the above described HPLC-PDA-ESI-tQ-MS conditions (Section 2.7) and full scan MS peak area used for calculation. One hundred and one compounds were quantitatively analyzed using 55 reference standards (Table S1). Each compound was carefully weighed (10 mg), dissolved in the methanol-DMSO mixture (1:1) in volumetric flasks (10 mL), and the reference standard calibration curves were built using the stock solutions in methanol (1–100 µg/mL). Mass spectrometric peak area data were used to plot ‘concentration–peak area’ graphs and determination the validation criteria (correlation coefficients, r2; standard deviation, SYX; limits of detection, LOD; limits of quantification, LOQ; and linear ranges) calculated as described previously [35] (Table S2). All quantitative analyses were carried out five times, and the data were expressed as mean value ± standard deviation (S.D.).

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