Microplate spectrophotometric assays were used to study the scavenging activity of R. adamsii extracts against 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH) [25], 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radicals (ABTS+) [26], N,N-dimethyl-p-phenylenediamine radicals (DMPD•+) [27], superoxide anion radicals (O2) [28], hydroxyl radicals (OH) [28], and chloride radicals (Cl) [29]. Carotene bleaching spectrophotometric assay used β-carotene as a substrate (Sigma-Aldrich, cat. No. C9750) [30] and nitric oxide (II) scavenging assay used sodium nitroprusside as NO source [31]. Ferrous (II) ion chelating activity was studied by spectrophotometric assay [32]. Trolox (cat. No. 238813, ≥97%; Sigma-Aldrich) was used as a reference standard (1–100 μg/mL in methanol). All the analyses were carried out five times and the data were expressed as mean value ± standard deviation (S.D.).

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