GC-MS measurement was performed as described (20), using an Agilent 7890B gas chromatograph equipped with a 30 m DB-35ms and 5 m Duraguard capillary column (Agilent, Santa Clara, California) for separation of derivatized metabolites, and an Agilent 5977B MSD system (Agilent) for measurement of metabolites, followed with data processing using the Metabolite Detector software (21).

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