2.7.4. Purification of peanut allergen protein Ara h 1
This protocol is extracted from research article:
Effect of ultrasonic pre-treatment on Ara h 1 in peanut sprouts
Ultrason Sonochem, May 25, 2021; DOI: 10.1016/j.ultsonch.2021.105607

Anion exchange chromatography of peanut sprouts was performed with an AKTA pure chromatography system (GE) and a DEAE Fast Flow column (GE Healthcare Company). The column was equilibrated until the baseline became smooth. Then a protein sample (5% of the column volume) was loaded onto the column. By continuously increasing the concentration of sodium chloride (dissolved in Tris-HCl as an elution buffer) to 0.4 M at a flow rate of 1.0 ml/min, the eluted proteins were monitored at 280 nm and then collected in the receptor machine. SDS-PAGE was subsequently performed to identify the components.

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