Degreased peanut powder and protein extraction buffer (pH 8.0, 50 mM Tris HCl buffer) were mixed at a mass to volume ratio of 1:10, magnetically stirred at 4 °C for 2 h, and then centrifuged at 10,000 g and 4 °C for 30 min. The supernatant was collected and stored at −20 °C for future use.

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