Peanut kernels contain as much as 43%–52% fat. To reduce interference in subsequent experiments, the freeze-dried, powdered peanuts/peanut sprouts were defatted, and the protein was extracted. One gram of powder from each treatment was mixed with 10 ml acetone and stirred at 4 °C for 2 h. Mixtures were then centrifuged at 3000 g (TGL-10G, Shanghai Anting Scientific Instrument Factory Co., Ltd., China) for 10 min, and the precipitate was obtained. The precipitate typically required three to four rounds of degreasing until the supernatant was transparent. The precipitate was then naturally air dried in the fume hood.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.