In this study, we used OSCC cell lines, HCS2, HOC313, HOC621, HOC719PE and HOC719NE. HSC2 cells were provided by the Japanese Collection of Research Bioresources Cell Bank. HOC313, HOC621, HOC719PE and HOC719NE cells were provided from Prof. Kamata (Hiroshima University). We previously found that HOC719NE and HOC313 have EMT features including loss of E-cadherin expression21,22. They were maintained in Dulbecco’s Modified Eagle Medium (Nacalai tesque, Inc., Kyoto, Japan) supplemented with 10% heat-inactivated FBS (Invitrogen) under conditions of 5% CO2 in air at 37˚C.

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