Effect of Plant Polysaccharide Pectin on the Toxicities of Spore–ICP Mixtures of Bt Against P. xylostella

The spore–crystal mixtures of Bt HD1 and HD73 were prepared according to the method described in the section Effect of Plant Polysaccharide Pectin on the Biofilms Formed by Bt Cells in Different Growth Phases at Solid–Liquid Interfaces. The mixtures were resuspended in sterile ddH2O, and their spore numbers were determined by the viable counting. The mixtures were diluted with sterile ddH2O to prepare 1.7 × 1013 and 0.85 × 1013 CFU/mL of Bt HD1, and 2.65 × 1015 and 1.325 × 1015 CFU/mL of Bt HD73. Then, 0, 5, and 25 mg/mL of pectin were added to them. The bioassay of P. xylostella of the spore–ICP mixtures was performed as follows: uniformly sized leaves that were not sprayed with pesticides were rinsed with water or the spore-ICP mixtures for 10 s and dried in the air; the leaves were soaked for 5 min in the mixtures with 0.1% detergent and air-dried. Then, the leaves were placed in 90-mm plates with absorbent papers; 30 3rd-instar larvae were transferred to each treatment, and the plates were strictly sealed with two-layer absorbent papers; after incubation in a light incubator for 2 days at 25°C, the number of dead and alive larvae was used for the calculation of corrected mortality.

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