Overnight culture of Bt strain was 1% transferred to LB medium with shaking at 220 rpm at 30°C until the OD600 was ∼1.00. The culture was 1% transferred to 200 mL LB medium with or without 5 mg/mL pectin in a 250-mL flask and cultured at 30°C with shaking at 220 rpm. The OD600 values were measured with a spectrophotometer (Cuevas and Edwards, 2017).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.