Overnight culture of Bt strain was 1% transferred to LB medium with shaking at 220 rpm at 30°C until the OD600 was ∼1.00. The culture was 1% transferred to 200 mL LB medium with or without 5 mg/mL pectin in a 250-mL flask and cultured at 30°C with shaking at 220 rpm. The OD600 values were measured with a spectrophotometer (Cuevas and Edwards, 2017).

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