Effect of Plant Polysaccharide Pectin on the Biofilms Formed by Bt Cells in Different Growth Phases at Solid–Liquid Interfaces

The biofilm induction effect of plant polysaccharide pectin was significantly higher than that of plant polysaccharide xylan. Therefore, the follow-up researches were focused only on plant polysaccharide pectin. Bt strains HD1, HD73, Bt185, HBF18, Bt185, G033A, and KN11 that are currently used for industrial production were selected to test the effect of pectin on the biofilm formation of Bt logarithmic growth phase cells. Briefly, the overnight cultured Bt strains were incubated to their logarithmic growth phases. Then, they were 1% transferred to 200 mL LB liquid medium (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) with or without 5 mg/mL pectin in a 250-mL flask and incubated at 30°C for 72 h. Then, the biofilms were harvested, dried, and weighed for quantitative analysis. The Bt strains that can form stronger biofilms induced by pectin were selected to further test the influence of pectin in the biofilm formation of Bt sporangial phase cells.

The above method was referred to in order to determine the effect of pectin on the biofilm formation of Bt spore–ICP mixture. Briefly, the mixture was prepared by liquid fermentation. The overnight cultured Bt strains were 2% transferred to 50 mL 1/2 LB liquid medium in a 250-mL flask containing and cultured with shaking at 220 revolutions/min (rpm) at 30°C until 50% of the spores were released. The Bt fermentation broth was centrifuged at 5,000 rpm for 5 min and washed three times with 0.5 M NaCl and sterile ddH2O. And the pellets were diluted with LB medium to 108 colony-forming units (CFUs)/mL to prepare the Bt spore–ICP mixture.

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