The bioinformatics website RNAhybrid was used to predict the potential binding sites between MONC and miR-636. MONC wild-type and mutant dual-luciferase reporter vectors were purchased from Liaoning Baihaobio Biotech Co., Ltd. (Liaoning, China), and co-transfected with miR-636 agomir or NC (GenePharma, Shanghai). The bioinformatics website, miRDB, was used to predict the potential binding sites between miR-636 and GLCE. GLCE wild-type and mutant dual-luciferase reporter vectors were purchased from Liaoning Baihaobio Biotech Co., Ltd., and co-transfected with miR-636 agomir or NC. A dual-luciferase reporter gene detection system (Promega, Madison, WI, USA) was used to detect luciferase activity.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.