DNA oligo probes (GenePharma) labeled with FAM for MONC (5′FAM-CAATCAGTTCCTCACATATTAGTCTAAGTCCTCTTTATAATTGAAGAAACATTCTTGTACTGGCTTTATGGGCATTCTTTCAATACTTTCAATGTTAAGAGGCCTTATT-3′) and SA-Biotin for miR-636 (5′Biotin-TGCGGGCGGGACGAGCAAGCACA-3′) were used in the FISH assays, wherein the nuclei were counterstained with 4,6-diamidino-2-phenylindole. All procedures were performed in accordance with the manufacturer’s instructions (GenePharma), and all images were acquired using a confocal laser-scanning microscope (CI: Nikon, Tokyo, Japan).

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