After cell transfection, 1 × 106 cells were collected from each group. After washing once in PBS, cells were stained with PE Annexin V and 7AAD using PE Annexin V Apoptosis Detection kit (BD Pharmingen™, New Jersey, USA) at room temperature for 15 min. We used flow cytometry (BD FACSCalibur, New Jersey, USA) to evaluate the proportion of apoptotic cells.

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