To build the structure of SARS-CoV-2 Cap(0)-RTC complex, we started with the model of the SARS-CoV-2 nsp12 and nsp7-8 complex (PDB: 7BTF), SARS-CoV-2 nsp13 (PDB: 6ZSL), nsp9 (PDB: 6W9Q) and nsp10/nsp14 (PDB: 6C8S). These were individually placed and rigid-body fitted into the cryo-EM map using UCSF Chimera(Pettersen et al., 2004). The model was manually built in Coot(Emsley et al., 2010) with the guidance of the cryo-EM map, and with real space refinement using Phenix(Afonine et al., 2018). The data validation statistics are shown in Table S1.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.