The binding reaction buffer contained 50 mM HEPES, pH 7.0, 100 mM NaCl, 2 mM MgCl2, 2 mM GDP and 2 mM BeF3 -. 18 μg RdRp (nsp12-nsp7-nsp8) complex protein was combined with 1.5 μg template-primer RNA, and RdRp/RNA and nsp13, or nsp9, or nsp10/14 mixed in a 1:2, or 1:1.2, or 1:1.2 molar ratio. Binding reactions were incubated for 30 min at 30°C. Reactions were run on a six lane polyacrylamide native gel (37.5:1 acrylamide:bis-acrylamide) running in 1 × TBE buffer at 150 V for 1h in 4°C. The gel was stained with ethidium bromide.

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